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Methyl-β-cyclodextrin modulates thapsigargin-induced store-dependent Ca 2+ entry in macrophages

Methyl-β-cyclodextrin modulates thapsigargin-induced store-dependent Ca 2+ entry in macrophages

Z I Krutetskaya, L S Milenina, A A Naumova, S N Butov, V G Antonov, A D Nozdrachev

Dokl Biochem Biophys. 2017 Mar;473(1):88-90.

PMID: 28510132

Abstract:

Using Fura-2AM microfluorimetry, we have shown for the first time that preincubation of macrophages with methyl-β-cyclodextrin, inducing cholesterol extraction from membranes and raft disruption, leads to significant inhibition of thapsigargin-induced store-dependent Ca2+ entry in rat peritoneal macrophages. In contrast, macrophage treatment with methyl-β-cyclodextrin after Ca2+ entry mechanisms were activated by store depletion by thapsigargin application leads to potentiation of subsequent store-dependent Ca2+ entry. The results suggest that intact lipid rafts are necessary for the activation but not the maintenance of store-dependent Ca2+ entry in macrophages.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP128446366	Methyl-β-cyclodextrin		128446-36-6	Price

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