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Micellar Electrokinetic Chromatography of Aminoglycosides

Ulrike Holzgrabe, Stefanie Schmitt, Frank Wienen

Methods Mol Biol. 2016;1483:77-90.

PMID: 27645732

Abstract:

The components of the aminoglycosides, e.g., gentamicin, sisomicin, netilmicin, kanamycin, amikacin, and tobramycin, and related impurities of these antibiotics can be separated by means of micellar electrokinetic chromatography (MEKC). Derivatization with o-phthaldialdehyde and thioglycolic acid is found to be appropriate for these antibiotics. The background electrolyte was composed of sodium tetraborate (100 mM), sodium deoxycholate (20 mM), and β-cyclodextrin (15 mM) having a pH value of 10.0. This method is valid for evaluation of gentamicin, kanamycin, and tobramycin. It has to be adopted for amikacin, paromomycin, neomycin, and netilmicin.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP643798 Phthaldialdehyde Phthaldialdehyde 643-79-8 Price
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