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Micro-bore Titanium Housed Polymer Monoliths for Reversed-Phase Liquid Chromatography of Small Molecules

Micro-bore Titanium Housed Polymer Monoliths for Reversed-Phase Liquid Chromatography of Small Molecules

Ekaterina P Nesterenko, Pavel N Nesterenko, Damian Connolly, Flavie Lacroix, Brett Paull

J Chromatogr A. 2010 Apr 2;1217(14):2138-46.

PMID: 20189186

Abstract:

A new method for the fixation of polymethacrylate monoliths within titanium tubing of up to 0.8 mm I.D. for use as a chromatographic column under elevated temperatures and pressures is described. The preparation of butyl methacrylate-ethylene dimethacrylate-based monolithic stationary phases with desired porous structures was achieved within titanium tubing with pre-oxidised internal walls. The oxidised titanium surface was subsequently silanised with 3-trimethoxysilylpropyl methacrylate resulting in tight bonding of butyl methacrylate porous monolith to the internal walls, providing stationary phase stability at column temperatures up to 110 degrees C and at operating column pressure drops of >28 MPa. The titanium housed monoliths exhibited a uniform and dense porous structure, which provided peak efficiencies of up to 59,000 theoretical plates per meter when evaluated for the separation of small molecules in reversed-phase mode, under optimal conditions (achieved at 15 microL/min and temperature of 110 degrees C for naphthalene with a retention factor, k=0.58). The developed column was applied to the reversed-phase isocratic separation of a text mixture of pesticides.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS78103	Micro particles based on polymethacrylate			Price

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