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Microarray Analysis of P-Anisaldehyde-Induced Transcriptome of Saccharomyces Cerevisiae

Microarray Analysis of P-Anisaldehyde-Induced Transcriptome of Saccharomyces Cerevisiae

Lu Yu, Na Guo, Yi Yang, Xiuping Wu, Rizeng Meng, Junwen Fan, Fa Ge, Xuelin Wang, Jingbo Liu, Xuming Deng

J Ind Microbiol Biotechnol. 2010 Mar;37(3):313-22.

PMID: 20024600

Abstract:

p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum L. seeds, is a potential novel preservative. To reveal the possible action mechanism of p-anisaldehyde against microorganisms, yeast-based commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes in response to p-anisaldehyde. Quantitative real-time RT-PCR was performed for selected genes to verify the microarray results. We interpreted our microarray data with the clustering tool, T-profiler. Analysis of microarray data revealed that p-anisaldehyde induced the expression of genes related to sulphur assimilation, aromatic aldehydes metabolism, and secondary metabolism, which demonstrated that the addition of p-anisaldehyde may influence the normal metabolism of aromatic aldehydes. This genome-wide transcriptomics approach revealed first insights into the response of Saccharomyces cerevisiae (S. cerevisiae) to p-anisaldehyde challenge.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP123115	p-Anisaldehyde		123-11-5	Price

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