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Microarray Karyotyping of Maltose-Fermenting Saccharomyces Yeasts With Differing Maltotriose Utilization Profiles Reveals Copy Number Variation in Genes Involved in Maltose and Maltotriose Utilization

Microarray Karyotyping of Maltose-Fermenting Saccharomyces Yeasts With Differing Maltotriose Utilization Profiles Reveals Copy Number Variation in Genes Involved in Maltose and Maltotriose Utilization

E H Duval, S L Alves Jr, B Dunn, G Sherlock, B U Stambuk

J Appl Microbiol. 2010 Jul;109(1):248-59.

PMID: 20070441

Abstract:

Aims:
We performed an analysis of maltotriose utilization by 52 Saccharomyces yeast strains able to ferment maltose efficiently and correlated the observed phenotypes with differences in the copy number of genes possibly involved in maltotriose utilization by yeast cells.
Methods and results:
The analysis of maltose and maltotriose utilization by laboratory and industrial strains of the species Saccharomyces cerevisiae and Saccharomyces pastorianus (a natural S. cerevisiae/Saccharomyces bayanus hybrid) was carried out using microscale liquid cultivation, as well as in aerobic batch cultures. All strains utilize maltose efficiently as a carbon source, but three different phenotypes were observed for maltotriose utilization: efficient growth, slow/delayed growth and no growth. Through microarray karyotyping and pulsed-field gel electrophoresis blots, we analysed the copy number and localization of several maltose-related genes in selected S. cerevisiae strains. While most strains lacked the MPH2 and MPH3 transporter genes, almost all strains analysed had the AGT1 gene and increased copy number of MALx1 permeases.
Conclusions:
Our results showed that S. pastorianus yeast strains utilized maltotriose more efficiently than S. cerevisiae strains and highlighted the importance of the AGT1 gene for efficient maltotriose utilization by S. cerevisiae yeasts.
Significance and impact of the study:
Our results revealed new maltotriose utilization phenotypes, contributing to a better understanding of the metabolism of this carbon source for improved fermentation by Saccharomyces yeasts.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1109280-A	Maltotriose		1109-28-0	Price

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