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Microbial Conversion of 5-sulfoisophthalic Acid Into 5-hydroxyisophthalic Acid by Ochrobactrum Anthropi S9

Microbial Conversion of 5-sulfoisophthalic Acid Into 5-hydroxyisophthalic Acid by Ochrobactrum Anthropi S9

Mamoru Yamada, Toyokazu Yoshida, Toru Nagasawa

Biotechnol Lett. 2010 Mar;32(3):445-50.

PMID: 19941033

Abstract:

5-Hydroxyisophthalic acid-producing microorganisms were isolated from enrichment cultures using 5-sulfoisophthalic acid as a sulfur source. One bacterium, Ochrobactrum anthropi S9, had the highest 5-sulfoisophthalic acid-degrading activity, and stoichiometrically formed 5-hydroxyisophthalic acid, a raw material for polymer synthesis. Under optimum culture conditions, 1.3 mM 5-hydroxyisophthalic acid accumulated in the medium by 60 h. The addition of Na(2)SO(4), L: -methionine or L: -cysteine at 2 mM inhibited the conversion of 5-sulfoisophthalic acid. O. anthropi S9 cells converted 5-sulfoisophthalic acid, benzenesulfonic acid, 3-sulfobenzoic acid, 4-aminobenzenesulfonic acid, naphthalene-1-sulfonic acid and naphthalene-2-sulfonic acid into the corresponding hydroxylated compounds.

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