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Microbial Interactions for Enhancement of α-amylase Production by

Microbial Interactions for Enhancement of α-amylase Production by

Bertrand Tatsinkou Fossi, Frédéric Tavea, Lum Ayeoffe Fontem, Robert Ndjouenkeu, Samuel Wanji

Biotechnol Rep (Amst). 2014 Sep 18;4:99-106.

PMID: 28626668

Abstract:

Interactions occurring between Saccharomyces cerevisiae and two thermostable α-amylase producing strains (Bacillus amyloliquefaciens 04BBA15 and Lactobacillus fermentum 04BBA19) were analyzed by comparing their growth patterns obtained in isolation with those obtained in mixture. The difference between the patterns was assessed using analysis of variance (ANOVA) in order to measure how much the growth of an organism was affected by other. The results showed two types of interactions in mixed culture; commensalism between S. cerevisiae and B. amyloliquefaciens 04BBA15 and mutualism between S. cerevisiae and L. fermentum 04BBA19. In mixed culture, the α-amylase production increased significantly compared to that observed in monoculture (P < 0.05). Response surface optimization of fermentation parameters in mixed cultures (initial yeast to bacteria ratio 1.125, temperature 33.5 °C, pH 5.5) resulted in about 1.8 fold higher enzyme production than that observed in the unoptimized fermentation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9000855-B	α-Amylase from Bacillus amyloliquefaciens		9000-85-5	Price

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