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Microdialysis of Melatonin in the Confluens Sinuum of the Rat Following Quantification by Gas Chromatography-Mass Spectrometry in the Negative Chemical Ion Mode

Microdialysis of Melatonin in the Confluens Sinuum of the Rat Following Quantification by Gas Chromatography-Mass Spectrometry in the Negative Chemical Ion Mode

H Boudra, S Saivin, P Delagrange, M F Malmary, P Genissel, G Houin

Arzneimittelforschung. 1999 Oct;49(10):849-52.

PMID: 10554663

Abstract:

In this study, an original surgical implantation technique in the confluens sinuum via the superior sagittal vein was developed to quantify melatonin secretion by the pineal gland. Melatonin (CAS 73-31-4) was determined using gas chromatography couples to negative ion chemical ionisation mass spectrometry following liquid extraction and derivatisation by penta-fluoropropionic acid anhydride (PFPA). The minimum detectable amount was 40 fg per injection, corresponding to 1 pg.ml-1 in dialysate. The assay was linear in the range 20-1000 pg.ml-1. This method was suitable for routine melatonin determination in dialysats of peripheral and central circulation with coefficients of variation of 11.2 and 24.6%, respectively for within and between analyses. Profiles of melatonin concentration were obtained (n = 3 rats) over a 2-day experimentation with a slowly diminution of the filtration capacity of the probe during the second day. The nocturnal concentrations of melatonin in the confluens sinuum dialysat ranged from 1003.9 to 2345 pg.ml-1 in the dialysat, indicating wide interindividual variations in the melatonin levels.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP73314-A	Melatonin - CAS 73-31-4		73-31-4	Price

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