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Microwave-assisted Extraction and Determination of Citrus Red 2 Dye in Oranges and Orange Juice by Liquid Chromatography-Tandem Mass Spectrometry

Microwave-assisted Extraction and Determination of Citrus Red 2 Dye in Oranges and Orange Juice by Liquid Chromatography-Tandem Mass Spectrometry

Chao Han, Bin Liu, Zhenou Zhu, Fuzhen Huang, Xiangzhun Chen, Yan Shen

J Food Sci. 2012 Dec;77(12):C1269-72.

PMID: 23171446

Abstract:

A fast, simple, low cost, and high-throughput method has been developed for the determination of citrus red 2 dye in orange and orange juice samples. The procedure is based on microwave-assisted extraction followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The method was optimized, and the analyte was efficiently extracted from the samples in 30 min using hexane/acetone (v/v, 3 : 1). The method was validated and showed good linearity and selectivity. The limits of quantification (LOQs) were 5 μg/kg (sample size of 2 g) for both orange and orange juice samples. The average recoveries, measured at 3 concentration levels (5, 10, and 20 μg/kg), were in the range 77.5% to 87.6% for the compound tested with relative standard deviations below 7.3%. The proposed method is rapid, accurate, and could be utilized for the routine analysis of citrus red 2 dye in orange and orange juice samples.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP6358538	Citrus Red 2		6358-53-8	Price

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