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MiRNA Quantitation With Microelectrode Sensors Enabled by Enzymeless Electrochemical Signal Amplification

MiRNA Quantitation With Microelectrode Sensors Enabled by Enzymeless Electrochemical Signal Amplification

Tanyu Wang, Gangli Wang, Didier Merlin, Emilie Viennois

Methods Mol Biol. 2017;1580:249-263.

PMID: 28439838

Abstract:

Quantification of circulating microRNAs (miRNAs) is of great interest because of their potentials as disease biomarkers. Currently, quantitative reverse transcription polymerase chain reaction (qRT-PCR) and microarray are considered mainstream techniques for miRNA identification and quantitation. However, these techniques are challenged by the low levels and wide dynamic range (from aM to nM) of miRNAs in a physiological sample, as well as the difficulty in the implementation in point-of-care settings. Here, we describe a one-step label-free electrochemical sensing technique by assembling a triple-stem DNA-redox probe structure on a gold microelectrode and introducing a reductant, tris(2-carboxyethyl) phosphine hydrochloride (TCEP) in the detection buffer solution to achieve ultrasensitive miRNAs detection with a detection limit of 0.1 fM.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP51805459-A	Tris(2-carboxyethyl)phosphine hydrochloride solution		51805-45-9	Price

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