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Mitochondrial Activity as an Indicator of Fish Freshness

Mitochondrial Activity as an Indicator of Fish Freshness

Jérôme Cléach, Philippe Pasdois, Philippe Marchetti, Denis Watier, Guillaume Duflos, Emmanuelle Goffier, Anne-Sophie Lacoste, Christian Slomianny, Thierry Grard, Philippe Lencel

Food Chem. 2019 Jul 30;287:38-45.

PMID: 30857714

Abstract:

The current methods used to routinely assess freshness in the fishing industry reflect more a state of spoilage than a state of freshness. Mitochondria, the seat of cellular respiration, undergo profound changes in post mortem tissues. The objective of this study was to demonstrate that mitochondrial activity constitutes a putative early fish freshness marker. The structure of gilthead sea bream (Sparus aurata) muscle tissue was evaluated over time by transmission electron microscopy. Respiration was assessed in mitochondria isolated from sea bream fillets using oxygraphy. Membrane potential (ΔΨm) was determined by fluorescence (Rhodamine 123). Mitochondrial activity of fillets stored at +4 °C was studied for 6 days. Changes in mitochondrial cristae structure appeared from Day 3 highlighting the presence of dense granules. ΔΨm and mitochondrial activity were significantly disrupted in sea bream fillets after 96 h of storage at +4 °C. Mitochondrial activity constituted a reliable and early indicator of fish freshness.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
ALP77228718	Carboxyatractyloside potassium salt		77228-71-8 (free acid)	Price

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