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Mitogenic Effect of Gastrin and Expression of Gastrin Receptors in Duct-Like Cells of Rat Pancreas

Mitogenic Effect of Gastrin and Expression of Gastrin Receptors in Duct-Like Cells of Rat Pancreas

I Rooman, J Lardon, D Flamez, F Schuit, L Bouwens

Gastroenterology. 2001 Oct;121(4):940-9.

PMID: 11606507

Abstract:

Background & aims:
Ductular metaplastic cells are observed during pancreas injury. Growth control by gastrin and expression of gastrin/cholecystokinin (CCK) B receptors were evaluated in these cells.
Methods:
Acinoductal transdifferentiation was induced in vitro by culturing of acinar cells, and ductular metaplasia was obtained in vivo by ligation of the pancreatic ducts. Mitogenic effects of gastrin I on ductal complexes in vivo and of tetragastrin, pentagastrin, and gastrin I and II, with or without the CCK-B receptor antagonist L-365,260, on duct-like cells in vitro were analyzed by 5-bromo-2'-deoxyuridine labeling. Immunocytochemistry, Western blotting, and reverse-transcription polymerase chain reaction were applied for detection of the CCK-B receptor.
Results:
Gastrin analogues induced a mitogenic stimulus in the duct-like cells in vitro and in ductal complexes in duct-ligated rat pancreas. Immunocytochemistry showed expression of CCK-B receptors in these models and in fetal but not normal adult exocrine pancreas. Additionally, up-regulation of CCK-B receptors during ductular metaplasia was shown by Western blotting and reverse-transcription polymerase chain reaction.
Conclusions:
Duct-like pancreatic epithelial cells in vitro and ductal complexes in vivo express gastrin/CCK-B receptors and proliferate in response to gastrin.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP81123060	Gastrin I rat		81123-06-0	Price

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