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Mixed Monolayer Decorated SPR Sensing Surface for Thrombin Detection

Mixed Monolayer Decorated SPR Sensing Surface for Thrombin Detection

Demet Ataman Sadık, İsmail Hakkı Boyacı, Mehmet Mutlu

J Pharm Biomed Anal. 2019 Nov 30;176:112822.

PMID: 31454662

Abstract:

The development of surface plasmon resonance (SPR) based immunosensor for thrombin detection was aimed. For this purpose, 3,3' Dithiodipropionic acid di (N-hydroxysuccinimide ester) (DSP):6-mercapto-1-hexanol (MCH) mixed self-assembled monolayers (mSAMs) were formed on gold surfaces for immobilization of anti-thrombin antibody. The performance of the immunosensor was determined against the target protein thrombin at various concentrations using flow cell coupled SPR. The linear detection range of the immunosensor was 30.0-100.0 nM with an R2 value of 0,992. Limit of Detection (LOD) and Limit of Quantification (LOQ) were determined to be 6.0 nM and 30.0 nM, respectively. The selectivity of the immunosensor was tested against a non-target model protein, human serum albumin (HSA) and the obtained ΔRU value was found to be below the ΔRU value corresponding to the LOQ concentration for thrombin. The immunosensor's capability to detect thrombin in diluted complex serum matrix was also tested and the obtained ΔRU value (159 ± 16) was compared with ΔRU value obtained for thrombin detection in PBS solution (137 ± 19). Based on the results, it was shown that DSP:MCH interface is a promising immobilization platform for binding biological recognition elements for the development of biosensors.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP57757570	3,3'-Dithiodipropionic acid di(N-hydroxysuccinimide ester)		57757-57-0	Price

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