Home
Resources
Publications
Modification of Materials Formed From poly(L-lactic Acid) to Enable Covalent Binding of Biopolymers: Application to High-Density Three-Dimensional Cell Culture in Foams With Attached Collagen

Modification of Materials Formed From poly(L-lactic Acid) to Enable Covalent Binding of Biopolymers: Application to High-Density Three-Dimensional Cell Culture in Foams With Attached Collagen

J Zheng, S R Northrup, P J Hornsby

In Vitro Cell Dev Biol Anim. 1998 Oct;34(9):679-84.

PMID: 9794219

Abstract:

We describe a method for increasing the hydrophilicity of materials formed from biodegradable polymers and introducing chemical functional groups on their surfaces. Poly(L-lactic acid) was blended with poly(epsilon-CBZ-L-lysine) at an 80:20 ratio. Films of the mixture were prepared and foams were made by solvent casting and salt leaching. Amino groups on the surface of the polymer mixture were deprotected by acid hydrolysis. As an example of the applicability of the technique for attachment of biomolecules, we covalently linked collagen to the deprotected amino groups, creating a surface capable of high density growth of a differentiated cell type (bovine adrenocortical cells). The method should be generally useful for surface modification of biodegradable polymer materials used in tissue engineering.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25931479	Poly-ε-Cbz-L-lysine		25931-47-9	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: