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Modified Nanoprecipitation Method to Fabricate DNA-loaded PLGA Nanoparticles

Modified Nanoprecipitation Method to Fabricate DNA-loaded PLGA Nanoparticles

Xiuming Niu, Weiwei Zou, Chunxi Liu, Na Zhang, Chunhua Fu

Drug Dev Ind Pharm. 2009 Nov;35(11):1375-83.

PMID: 19832638

Abstract:

Objective:
The objective of this study was to formulate DNA-loaded poly(d,l-lactide-co-glycotide) (PLGA) nanoparticles by a modified nanoprecipitation method.
Methods:
DNA-loaded PLGA nanoparticles were prepared by the modified nanoprecipitation method and the double emulsion/solvent evaporation method. The characterizations of DNA-loaded nanoparticles such as entrapment efficiency, morphology, particle size, zeta potential, structural integrity of the loaded DNA, and stability of the loaded DNA in PLGA nanoparticles against DNase I, in vitro release, cell viability and in vitro transfection capability were investigated.
Results:
The resulted PLGA nanoparticles by the modified nanoprecipitation method had uniform spherical shape, narrow size distribution with average particles size near 200 nm, negative zeta potential of -12.6 mV at pH 7.4, and a sustained-release property in vitro. Plasmid DNA could be efficiently encapsulated into PLGA nanoparticles (> 95%) without affecting its intact conformation using this modified nanoprecipitation method, which was superior to the double emulsion/solvent evaporation method. The PLGA nanoparticles were much safer to A549 cell compared to commercial Lipofectamine 2000 and could successfully transfer plasmid-enhanced green fluorescent protein into A549 cells.
Conclusion:
In conclusion, the modified nanoprecipitation method could be applied as an efficient way to fabricate DNA-loaded PLGA nanoparticles instead of the conventional double emulsion/solvent evaporation method.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS743137	Green Fluorescent PLGA nanoparticles			Price

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