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Modified Radical Scavenging and Antioxidant Activity Measurement of β-Carotene With β-Cyclodextrins Complexation in Aqueous Medium

Modified Radical Scavenging and Antioxidant Activity Measurement of β-Carotene With β-Cyclodextrins Complexation in Aqueous Medium

Saliha Esin Çelik, Burcu Bekdeser, Ayse Nur Tufan, Resat Apak

Anal Sci. 2017;33(3):299-305.

PMID: 28302970

Abstract:

In order to evaluate the antioxidant capacity/activity of β-carotene (BC) in aqueous media, we investigated the inclusion complexes of BC with methyl-β-cyclodextrin (Me-β-CD), 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) and 2-hydroxyethyl-β-cyclodextrin (HE-β-CD) that enhance water solubility and chemical stability. The inclusion complexes (monitored by FTIR) exhibited higher solubility than free BC, and phase solubility studies showed a linear increase in the solubility with the Me-β-CD concentration. Cupric ion-reducing antioxidant capacity (CUPRAC), ABTS-persulfate, peroxyl and hydroxyl radical scavenging assays were applied. CD-complexed β-carotene exhibited less effective antioxidative and radical scavenging than free BC dissolved in acetone. β-Carotene showed the highest antioxidant capacity in the presence of HE-β-CD, and the lowest with Me-β-CD, probably due to the deeper and more hydrophobic cavity of the latter. We believe that this is the first report on devising simple spectrophotometric methods for the wholistic assessment of antioxidant activity/capacity, hydroxyl and peroxyl radical scavenging activity of β-carotene in aqueous solution with CDs.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP128446322	(2-Hydroxyethyl)-β-cyclodextrin		128446-32-2	Price

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