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Modulation of Apoptosis Protein Profiles--Role of P-gp in HeLa Cells Exposed to Doxorubicin

Modulation of Apoptosis Protein Profiles--Role of P-gp in HeLa Cells Exposed to Doxorubicin

Beata M Gruber, Jolanta Krzysztoń-Russjan, Irena Bubko, Mirosława Koronkiewicz, Elzbieta L Anuszewska

Cancer Genomics Proteomics. May-Jun 2012;9(3):153-61.

PMID: 22593250

Abstract:

As shown previously doxorubicin (1 μM) plus sulindac (50 μM) reduced the expression of ABCB1 (ATP-binding cassette, sub-family B (MDR/TAP), member 1) mRNA in HeLa cells and this effect was accompanied by increased apoptosis. The aim of this study was to define if the decrease of ABCB1 expression or blocking of P-glycoprotein (P-gp) can affect the expression of the apoptotic genes determined with use of quantitative real time polymerase chain reaction (qRT-PCR). Western blot was used for visualization of chosen pro- and antiapoptotic proteins. Doxorubicin was the main compound which affected the apoptotic genes. The effectiveness of the drugs in reducing of P-gp function has been shown as not being related to the regulation of apoptotic gene transcription. In this experimental scheme, regulation of apoptotic gene transcription depended on the kind of P-gp modulator.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP59973807	Sulindac Related Compound B		59973-80-7	Price

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