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Modulation of Solvation and Molecular Recognition of a Lipid Bilayer Under Dynamical Phase Transition

Modulation of Solvation and Molecular Recognition of a Lipid Bilayer Under Dynamical Phase Transition

Priya Singh, Susobhan Choudhury, V K Sharma, S Mitra, R Mukhopadhyay, Ranjan Das, Samir Kumar Pal

Chemphyschem. 2018 Oct 19;19(20):2709-2716.

PMID: 30030893

Abstract:

It is well accepted in contemporary biology that an ∼30 Å thick lipid bilayer film around living cells is a matter of life and death as the film typically delimits the environments that serve as a crucial margin. The dynamic organization of lipid molecules both across the lipid bilayer and in the lateral dimension are known to be crucial for cellular transport and molecular recognition by important biological macromolecules. Here, we study dilute (20 mM) Dioctadecyldimethylammonium bromide (DODAB) vesicles at different temperatures in aqueous dispersion with well-defined phases namely liquid crystalline, gel and subgel. The spectroscopic studies on two fluorescent probes 8-anilino-1-naphthalene sulfonic acid ammonium salt (ANS) and Coumarin 500 (C500), former in the head group region of the lipid-water interface and later located deeper in the lipid bilayer follow dynamics (solvation and fluidity) of their local environments in the vesicles. Binding of an anti-tuberculosis drug rifampicin has also been studied employing Förster resonance energy transfer (FRET) technique. The molecular insight concerning the effect of dynamical organization of the lipid molecules on the local dynamics of aqueous environments in different phases leading to molecular recognition becomes evident in our study.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP84806279	7-Fluorobenzofurazan-4-sulfonic acid ammonium salt		84806-27-9	Price

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