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Molecular Cloning, Purification and Functional Implications of Recombinant GST Tagged hGMCSF Cytokine

Molecular Cloning, Purification and Functional Implications of Recombinant GST Tagged hGMCSF Cytokine

Nidhi Chaubey, Siddhartha Sankar Ghosh

Appl Biochem Biotechnol. 2013 Mar;169(5):1713-26.

PMID: 23334834

Abstract:

We report the cloning, purification and cell proliferative activity of a novel recombinant GST tagged human granulocyte macrophage colony stimulating factor (GST-hGMCSF). The hGMCSF gene was PCR amplified from the cDNA of ACHN renal carcinoma cells and was cloned into the bacterial expression vector. The GST-hGMCSF was purified to homogeneity using glutathione agarose affinity chromatography and subsequently characterized by Western blot, circular dichroism (CD) and MALDI TOF-TOF analysis. Homology modelling studies revealed the possible binding domains of the recombinant cytokine with cognate receptor. The proliferation of THP-1, Raw 264.7, MCF-7 and U87MG cells upon GST-hGMCSF addition was found to be dose dependent. Hence, this functionally active recombinant cytokine has potential application in cancer therapy for stimulating facile growth recovery of normal cell population.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411820	CAMK1δ, active, GST tagged human			Price
IAR42415012	P73b, GST tagged human			Price

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