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Molecular Genetic and Biochemical Responses in Human Airway Epithelial Cell Cultures Exposed to Titanium Nanoparticles in Vitro

Molecular Genetic and Biochemical Responses in Human Airway Epithelial Cell Cultures Exposed to Titanium Nanoparticles in Vitro

Elanur Aydın, Hasan Türkez, Fazıl Hacımüftüoğlu, Abdulgani Tatar, Fatime Geyikoğlu

J Biomed Mater Res A. 2017 Jul;105(7):2056-2064.

PMID: 28028929

Abstract:

Titanium nanoparticles (NPs) have very wide application areas such as paint, cosmetics, pharmaceuticals, and biomedical applications. And, to translate these nanomaterials to the clinic and industrial domains, their safety needs to be verified, particularly in terms of genotoxicity and cytotoxicity. Therefore, in this study, we aimed to investigate of cytotoxicity and changes in gene expression profiles influenced by commonly titanium (as titanium carbide, titanium carbo-nitride, titanium (II) oxide, titanium (III) oxide, titanium (IV) oxide, titanium nitride, titanium silicon oxide) NPs in human alveolar epithelial (HPAEpiC) and pharynx (HPPC) cell lines in vitro since inhalation is an important pathway for exposure to these NPs. HPAEpiC and HPPC cells were treated with titanium (0-100 µg/mL), NPs for 24 and 48 h, and then cytotoxicity was detected by, [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT), uptake of neutral red (NR) and lactate dehydrogenase (LDH) release assays, while genotoxicity was also analyzed by cDNA array - RT-PCR assay. According to the results of MTT, NR and LDH assays, all tested NPs induced cytotoxicity on both HPAEpiC and HPPC cells in a time- and dose-dependent manner. Determining and analyzing the gene expression profiles of HPAEpiC and HPPC cells, titanium NPs showed more changes in genes related to DNA damage or repair, oxidative stress, and apoptosis. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2056-2064, 2017.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP12137201	Titanium(II) oxide		12137-20-1	Price

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