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Monobody-Mediated Alteration of Lipase Substrate Specificity

Monobody-Mediated Alteration of Lipase Substrate Specificity

Shun-Ichi Tanaka, Tetsuya Takahashi, Akiko Koide, Riki Iwamoto, Satoshi Koikeda, Shohei Koide

ACS Chem Biol. 2018 Jun 15;13(6):1487-1492.

PMID: 29757606

Abstract:

Controlling the catalytic properties of enzymes remain an important challenge in chemistry and biotechnology. We have recently established a strategy for altering enzyme specificity in which the addition of proxy monobodies, synthetic binding proteins, modulates the specificity of an otherwise unmodified enzyme. Here, in order to examine its broader applicability, we employed the strategy on Candida rugosa lipase 1 (CRL1), an enzyme with a tunnel-like substrate binding site. We successfully identified proxy monobodies that restricted the substrate specificity of CRL1 toward short-chain fatty acids. The successes with this enzyme system and a β-galactosidase used in the previous work suggest that our strategy can be applied to diverse enzymes with distinct architectures of substrate binding sites.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP195833466	Lipase Substrate		195833-46-6	Price

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