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Monoclonal Antibody to Blood Group Glycosyltransferases, Produced by Hybrids Constructed With Epstein-Barr-virus-transformed B Lymphocytes From a Patient With ABO-incompatible Bone Marrow Transplant and Mouse Myeloma Cells

Monoclonal Antibody to Blood Group Glycosyltransferases, Produced by Hybrids Constructed With Epstein-Barr-virus-transformed B Lymphocytes From a Patient With ABO-incompatible Bone Marrow Transplant and Mouse Myeloma Cells

Y Kominato, T Fujikura, I Shimada, H Takizawa, K Hayashi, T Mori, T Matsuda

Vox Sang. 1990;59(2):116-8.

PMID: 2173279

Abstract:

A patient with chronic myeloid leukemia secreted an antibody to blood group glycosyltransferases after ABO-incompatible bone marrow transplantation (B recipient/O donor). Peripheral B lymphocytes from the recipient were transformed with Epstein-Barr virus, and then fused by polyethylene glycol with mouse myeloma cell line P3-X63/Ag8.653. After the cloning of the hybridoma cells, a cell line which produced human IgM antibody to blood group glycosyltransferases was established. The antibody completely neutralized B transferase activity at low concentration, while a larger amount of immunoglobulins was required to neutralize A transferase activity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414528	Monoclonal Anti-RAP1 A/B antibody produced in mouse			Price

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