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Monoclonal Antibody to the Alpha 1-subunit of the Dihydropyridine-Binding Complex Inhibits Calcium Currents in BC3H1 Myocytes

Monoclonal Antibody to the Alpha 1-subunit of the Dihydropyridine-Binding Complex Inhibits Calcium Currents in BC3H1 Myocytes

M E Morton, J M Caffrey, A M Brown, S C Froehner

J Biol Chem. 1988 Jan 15;263(2):613-6.

PMID: 2447079

Abstract:

A monoclonal antibody, mAb 1A, previously shown to recognize the dihydropyridine (DHP)-binding complex in rabbit muscle transverse tubules, inhibits voltage-sensitive calcium channel activity in a mouse muscle cell line. mAb 1A applied externally to BC3H1 muscle cells produced a concentration-dependent attenuation of the slowly-activating, DHP-sensitive, high-threshold calcium current. mAb 1A had no direct effect on either the rapidly-activating, DHP-insensitive, low-threshold calcium current or the delayed outward potassium current. However, sodium current kinetics were altered. On Western blot analysis of immunoaffinity-purified protein solubilized from BC3H1 membranes, mAb 1A recognized a Mr 210,000 polypeptide that is an analogous to the alpha 1 subunit of the DHP-binding complex from skeletal muscle. These results suggest that an extracellular segment of the alpha 1 subunit of the DHP-binding complex plays a functionally important role in voltage-gated calcium currents.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42417109	Anti-phospho-Nonvoltage-gated Sodium Channel 1 (pThr615) antibody produced in rabbit			Price

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