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Multicolor Fluorescent Labeling of Cellulose Nanofibrils by Click Chemistry

Multicolor Fluorescent Labeling of Cellulose Nanofibrils by Click Chemistry

Julien R G Navarro, Guillaume Conzatti, Yang Yu, Andreas B Fall, Renny Mathew, Mattias Edén, Lennart Bergström

Biomacromolecules. 2015 Apr 13;16(4):1293-300.

PMID: 25774999

Abstract:

We have chemically modified cellulose nanofibrils (CNF) with furan and maleimide groups, and selectively labeled the modified CNF with fluorescent probes; 7-mercapto-4-methylcoumarin and fluorescein diacetate 5-maleimide, through two specific click chemistry reactions: Diels-Alder cycloaddition and the thiol-Michael reaction. Characterization by solid-state (13)C NMR and infrared spectroscopy was used to follow the surface modification and estimate the substitution degrees. We demonstrate that the two luminescent dyes could be selectively labeled onto CNF, yielding a multicolor CNF that was characterized by UV/visible and fluorescence spectroscopies. It was demonstrated that the multicolor CNF could be imaged using a confocal laser scanning microscope.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP137215271	7-Mercapto-4-methylcoumarin		137215-27-1	Price

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