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N-(3-triethoxysilylpropyl)-4-(isothiocyanatomethyl)-cyclohexane-1-carboxamide (TPICC): A Heterobifunctional Reagent for Immobilization of Biomolecules on Glass Surface

N-(3-triethoxysilylpropyl)-4-(isothiocyanatomethyl)-cyclohexane-1-carboxamide (TPICC): A Heterobifunctional Reagent for Immobilization of Biomolecules on Glass Surface

Arvind Misra, Mohammad Shahid, Pratibha Dwivedi

Bioorg Med Chem Lett. 2008 Oct 1;18(19):5217-21.

PMID: 18783945

Abstract:

An efficient heterobifunctional reagent, N-(3-triethoxysilylpropyl)-4-(isothiocyanatomethyl)cyclohexane-1-carboxamide (TPICC) has been developed by a simple 'two step reaction' for the preparation of bioconjugates and immobilization of biomolecules such as oligonucleotides, peptides and proteins on the glass surface. The isothiocyanate functionality at one end of the reagent, TPICC was found specific for the ligands having either aminoalkyl (RNH(2)) or mercaptoalkyl (R-SH) functionality. The synthesis of bioconjugates with the reagent was achieved through its isothiocyanate functionality at one end via the formation of stable thiourea linkage with aminoalkyl and dithiocarbamate linkage with mercaptoalkyl derivatives. The triethoxysilyl functionality of the reagent has been utilized for specific coupling with the virgin glass surface by a very simple methodology.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP18151843	(Triethoxysilyl)cyclohexane		18151-84-3	Price

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