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N-hydroxysuccinimide Ester Labeling 5'-aminoalkyl DNA Oligomers: Reaction Conditions and Purification

Z Wang, N Bian, X Qian, R W Giese

J Chromatogr A. 1998 May 8;806(1):93-5.

PMID: 9639882

Abstract:

Difficulties were encountered in labeling 5'-aminoalkyl DNA oligomers with glycolketo electrophore N-hydroxysuccinimide esters in aqueous sodium bicarbonate (a common base for this purpose), followed by C18-silica reversed-phase high-performance liquid chromatography (HPLC) to achieve purification. The electrophore-labeled oligomers were not separated readily either from the hydrolyzed electrophore or from the starting oligomer. This problem was overcome by conducting the reaction with triethylamine as a base, organic washing the reaction mixtures after evaporation, and separating on a C18-poly(styrene-divinylbenzene) HPLC packing.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42418203 Fluorescein-5-EX N-hydroxysuccinimide ester Fluorescein-5-EX N-hydroxysuccinimide ester Price
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