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N-linked Oligosaccharide Structures in the Diamine Oxidase From Porcine Kidney

N-linked Oligosaccharide Structures in the Diamine Oxidase From Porcine Kidney

Y Huang, Y Mechref, M V Novotny

Carbohydr Res. 2000 Jan 12;323(1-4):111-25.

PMID: 10782293

Abstract:

Structures of the N-linked glycans released from porcine kidney diamine oxidase (DAO) were characterized utilizing various analytical techniques, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS), high-performance capillary electrophoresis (HPCE), and high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The oligosaccharide sequences present in DAO were conclusively determined using specific exoglycosidases in conjunction with MALDI/TOF-MS. The structures found in the glycoprotein are primarily linear, di-, or tribranched fucosylated complex type. MS analysis of the esterified N-glycan pool derived from DAO indicated the presence of several di- and trisialylated structures.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9001530	Diamine Oxidase from porcine kidney		9001-53-0	Price

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