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N-myristoyltransferase Inhibitor Protein Is Homologous to Heat Shock Cognate Protein 70

N-myristoyltransferase Inhibitor Protein Is Homologous to Heat Shock Cognate Protein 70

Ponniah Selvakumar, Ashakumary Lakshmikuttyamma, Mohammed Khysar Pasha, Martin J King, Douglas J H Olson, Sumiko Mori, Andrew R S Ross, Kiyoshi Hayashi, Jonathan R Dimmock, Rajendra K Sharma

J Cell Biochem. 2004 Jun 1;92(3):573-8.

PMID: 15156568

Abstract:

Many of viral and eukaryotic proteins are required for signal transduction and regulatory functions which undergo a lipid modification by the enzyme N-myristoyltransferase (NMT). In this study, we demonstrated that heat shock cognate protein 70 (HSC70) is homologous to NMT inhibitor protein (NIP71), which was discovered in our laboratory, based on MALDI-TOF mass spectrometric analysis. The purified bovine cytosolic HSC70 and particulate NIP71 produced a dose-dependent inhibition of human NMT having half maximal inhibitions of 235 and 230 nM, respectively. Further, Western blot analysis revealed that the purified particulate NIP71 and cytosolic HSC70 cross-reacted with both anti-NIP71 and anti-HSC70 antibodies. The results we obtained imply that molecular chaperones could be involved in the regulation of NMT in normal and cancerous cells. Further studies directed to revealing the role of HSC70 in the regulation of NMT may lead to the development of gene based therapies of colon cancer.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4241050	Heat Shock Cognate Protein 70 bovine			Price

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