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N-succinyl-alanyl-methionyl-S-benzylcysteine P-Nitroanilide as a Sensitive Substrate for Assaying Activity of Cysteine Proteinases

L Fukal, E Kasafírek

J Biochem Biophys Methods. 1988 Aug;16(4):323-5.

PMID: 3221040

Abstract:

N-Succinyl-alanyl-methionyl-S-benzylcysteine p-nitroanilide has been found to be a very sensitive chromogenic substrate for the assay of cysteine proteinase papain, ficin and bromelain. N-Succinyl-alanyl-S-benzylcysteine p-nitroanilide and N-succinyl-alanyl-alanyl-S-benzylcysteine p-nitroanilide are also suitable for this purpose. These substrates were hydrolyzed only very slightly or not hydrolyzed at all by trypsin.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP108322038 N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide 108322-03-8 Price
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