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Naphthol-yellow-S Protein Content of Individual Rat Hepatocytes as Related to Food Intake and Short-Term Starvation

Naphthol-yellow-S Protein Content of Individual Rat Hepatocytes as Related to Food Intake and Short-Term Starvation

J James, K S Bosch, G M Fronik, J M Houtkooper

Cell Tissue Res. 1986;243(1):165-9.

PMID: 3943120

Abstract:

With regard to the protein content, as analysed cytophotometrically, of hepatocytes from rats kept under a 12L 12D photoperiod (photophase 7:00-19:00), the following facts have been established: 1) Hepatocytes of different classes of ploidy all demonstrate, more or less equally, daily variations in protein content and also its reduction after 24-h fasting. 2) With computer analysis of data obtained at eight time points during a period of 24 h, a sinusoidal curve of the protein content of individual mononuclear tetraploid hepatocytes throughout the day could be demonstrated with a maximum at 6:20 and a minimum at 18:20. 3) Animals, fed with meals via a dispensing machine from 23:00 to 24:00 only, show a similar sinusoidal curve but with higher amplitude, and a virtually identical mean value as those fed ad libitum. The maximum was found at 10:40, revealing a time lag of 12 h after food intake, the minimum at 22:40. 4) Trained animals deprived of food during the standardized feeding time revealed a moderate reduction of their hepatocyte protein content in the first 6 h, then a 6-h period with a steep fall followed by a slower reduction. After 24 h, the mean hepatocyte protein mass had decreased to 72% of that at the commencement of fasting at 23:00.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP846708	Naphthol Yellow S		846-70-8	Price

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