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Negative Pressure Cavitation-Microwave Assisted Preparation of Extract of Pyrola Incarnata Fisch. Rich in Hyperin, 2'-O-galloylhyperin and Chimaphilin and Evaluation of Its Antioxidant Activity

Negative Pressure Cavitation-Microwave Assisted Preparation of Extract of Pyrola Incarnata Fisch. Rich in Hyperin, 2'-O-galloylhyperin and Chimaphilin and Evaluation of Its Antioxidant Activity

Xiao-Hui Yao, Dong-Yang Zhang, Meng Luo, Shuang Jin, Yuan-Gang Zu, Thomas Efferth, Yu-Jie Fu

Food Chem. 2015 Feb 15;169:270-6.

PMID: 25236226

Abstract:

A novel and effective extraction method, namely negative pressure cavitation-microwave assisted extraction technique (NMAE), was developed for the preparation of extracts of Pyrola incarnata Fisch., which are rich in the main constituents hyperin, 2'-O-galloylhyperin and chimaphilin. Single factor experiments and Box-Behnken design (BBD) were combined with a response surface methodology to examine factors affecting extraction. Maximum extraction yields of hyperin, 2'-O-galloylhyperin and chimaphilin (1.339±0.029, 4.831±0.117 and 0.329±0.011mg/g, respectively) were achieved under the following optimised conditions: 700W microwave power, 50°C extraction temperature, 30:1mL/g liquid-solid ratio, -0.05MPa negative pressure, 55% ethanol concentration and 12min extraction time. First-order kinetics equation demonstrated that NMAE offered significant savings in extraction time, and enhancing extraction efficiency. Furthermore, NMAE extracts yielded excellent antioxidant activity (IC50 0.121mg/mL for DPPH 2.896mmol FeSO4/g DW FRAP).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP53209271	2′′-Galloylhyperin		53209-27-1	Price

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