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[Neurotrophin 3 Promotes Osteogenic Differentiation of Human Dental Follicle Cells]

[Neurotrophin 3 Promotes Osteogenic Differentiation of Human Dental Follicle Cells]

Lei Wei, Ma Yue, Ren Aishu

Hua Xi Kou Qiang Yi Xue Za Zhi. 2018 Feb 1;36(1):23-28.

PMID: 29594991

Abstract:

Objective:
This study aims to investigate the effect of neurotrophin 3 (NT-3) on the osteogenic differentiation of human dental follicle cells (hDFCs).
Methods:
hDFCs were isolated and cultured in vitro. Immunocytochemical staining was used to identify the origin of hDFCs. The effects of different NT-3 concentrations on hDFCs proliferation were detected by using CCK-8 assay. The alkaline phosphatase (ALP) activities and mRNA expression levels of bone morphogenetic protein-2 (BMP-2) and osteocalcin (OCN) were determined to investigate the effects of NT-3 on hDFCs osteogenesis. The difference in the number of mineralized nodules was detected using alizarin red staining.
Results:
Vimentin and cytokeratin staining results showed that hDFCs originated from the mesenchymal cells. NT-3 exerted no evident effect on hDFCs proliferation. The ALP activity and the BMP-2 and OCN mRNA expression levels of hDFCs were signiﬁcantly improved under treatment with different NT-3 concentrations (25, 50, and 100 ng·mL ⁻¹) compared with those in the control group. BMP-2 and OCN mRNA relative expression levels of hDFCs reached the highest when the NT-3 concentration was 100 ng·mL ⁻¹. The number of mineralized nodules reached the maximum when the hDFCs were treated with 50 and 100 ng·mL ⁻¹ NT-3.
Conclusions:
Appropriate mass concentration of NT-3 can promote the osteogenic differentiation of hDFCs.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248624	Neurotrophin-3 human			Price

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