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Neutral Red Versus MTT Assay of Cell Viability in the Presence of Copper Compounds

Mariela Gomez Perez, Lyvia Fourcade, Mircea Alexandru Mateescu, Joanne Paquin

Anal Biochem. 2017 Oct 15;535:43-46.

PMID: 28778493

Abstract:

Copper is essential for numerous physiological functions, and copper compounds may display therapeutic as well as cytotoxic effects. The MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay is a standard test largely used in cytotoxicity studies. This report shows that low micromolar levels of copper compounds such as Cu(II)Urea2, Cu(II)Ser2 and CuCl2 can interfere with the MTT assay making improper the detection of formazan product of MTT reduction. Comparatively, the Neutral Red assay appears to be sensitive and showing no interference with these compounds. The lactate dehydrogenase alternative assay cannot be used because of inhibitory effect of these copper compounds on the enzyme activity.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP553242 Neutral Red Neutral Red 553-24-2 Price
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