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New Application of Silane Coupling Agents for Covalently Binding Antibodies to Glass and Cellulose Solid Supports

New Application of Silane Coupling Agents for Covalently Binding Antibodies to Glass and Cellulose Solid Supports

N M Pope, D L Kulcinski, A Hardwick, Y A Chang

Bioconjug Chem. Mar-Apr 1993;4(2):166-71.

PMID: 7873648

Abstract:

Bifunctional silane reagents (3-iodopropyl)trimethoxysilane (1), (gamma-glycidoxypropyl)trimethoxysilane (2), and [1-(trimethoxysilyl)-2-(m- (or p-)chloromethyl)phenyl]ethane (3) were used to covalently link goat anti-mouse (GAM) antibodies (Ab) to glass microbeads and cuprammonium rayon hollow-fiber dialyzers. An average of 0.79 and 0.83 microgram of GAM Ab/cm2 was immobilized on the hollow-fiber dialyzers and the glass beads, respectively. The antibodies immobilized on glass microbeads or on hollow-fiber dialyzers were then used to selectively deplete CD34+ cells or CD4+ peripheral blood mononuclear cells (PBMC), respectively. Glass microbeads depleted 80% CD34+ cells with good selectivity, and the hollow-fiber dialyzers depleted an average of 81% CD4+ PBMC.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP14867288	(3-Iodopropyl)trimethoxysilane		14867-28-8	Price

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