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New Laboratory Scale Purification of Beta-Amanitin From American Amanita Phalloides

R R Yocum

Biochemistry. 1978 Sep 5;17(18):3786-9.

PMID: 568002

Abstract:

A modern laboratory scale method for purifying beta-amanitin from the mushroom Amanita phalloides is described. The purification involves solubilizing the toxin from dried mushrooms, removing the lipids by chloroform extraction, desalting on Amberlite XAD-4, adsorption chromatography on Sephadex LH-20 at acidic and neutral pH, and ion-exchange chromatography on QAE-Sephadex. The resulting beta-amanitin is homogeneous by thin-layer chromatography and is biologically active.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP21150221 β-Amanitin from Amanita phalloides β-Amanitin from Amanita phalloides 21150-22-1 Price
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