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Nitroxides Prevent Protein Glycoxidation in Vitro

Nitroxides Prevent Protein Glycoxidation in Vitro

I Sadowska-Bartosz, S Galiniak, J Skolimowski, I Stefaniuk, G Bartosz

Free Radic Res. 2015 Feb;49(2):113-21.

PMID: 25363554

Abstract:

Seven nitroxides of different structures were studied for the ability to prevent glycoxidation of bovine serum albumin incubated with three monosaccharides (glucose, fructose, and ribose). Glycoxidation was estimated by fluorimetric parameters of protein modifications (formation of advanced glycation end products [AGEs], dityrosine, N'-formylkynurenine, and kynurenine) and enzyme-linked immunosorbent assay for AGEs. From among the nitroxides tested, (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO), 4-carboxy-TEMPO, and 4-hydroxy-TEMPO offered significant protection against glycoxidation induced by glucose and fructose, while 3-carbamoyl-PROXYL was not protective, enhancing glycoxidation. Lower protection was observed for glycoxidation induced by ribose where only 3-carbamoyl-PROXYL and 4-amino-TEMPO showed some protection. Loss of electron spin resonance signal of the nitroxides was observed during glycoxidation indicating occurrence of free radical reactions in this process. These results suggest for the first time that nitroxides may be promising compounds for preventing glycoxidation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP37149181	4-Carboxy-TEMPO, free radical		37149-18-1	Price

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