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NMR and Enzymatic Investigation of the Interaction Between Elastase and Sodium Trifluoroacetate

NMR and Enzymatic Investigation of the Interaction Between Elastase and Sodium Trifluoroacetate

C Winninger, P Lestienne, J L Dimicoli, J G Bieth

Biochim Biophys Acta. 1978 Sep 11;526(1):227-34.

PMID: 28776

Abstract:

At pH 5.5, sodium trifluoroacetate is a potent competitive inhibitor of porcine elastase (Ki = 2.6 mM) and human leukocyte elastase (Ki = 9.3 mM). For both enzymes the Ki increases strongly with pH. Sodium fluoride is inactive on pancreatic elastase and sodium acetate is a weak inhibitor of this enzyme. Trifluoroethanol inhibits both enzymes but is less active than trifluoroacetate in acidic pH conditions. Bovine trypsin and alpha-chymotrypsin are resistant to the action of sodium trifluoroacetate and trifluoroethanol. The interaction between sodium trifluoroacetate and pancreatic elastase is also demonstrated by 19F NMR spectroscopy. Trifluoroacetyltrialanine is able to displace trifluoroacetate from its complex with pancreatic elastase. In addition, a method using turkey ovomucoid for the active site titration of leukocyte and pancreatic elastase is described.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP2923184	Sodium trifluoroacetate		2923-18-4	Price

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