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Nonadhesive Nanotopography: Fibroblast Response to Poly(n-Butyl Methacrylate)-Poly(styrene) Demixed Surface Features

Nonadhesive Nanotopography: Fibroblast Response to Poly(n-Butyl Methacrylate)-Poly(styrene) Demixed Surface Features

M J Dalby, M O Riehle, H J H Johnstone, S Affrossman, A S G Curtis

J Biomed Mater Res A. 2003 Dec 1;67(3):1025-32.

PMID: 14613253

Abstract:

It is becoming clear that cells do not only respond to micrometric scale topography, but may also respond to topography at the nanometric scale. Nano-fabrication methods such as electron beam lithography are, however, expensive and time consuming. Polymer demixing of poly(styrene) and poly(4-bromostyrene) has been found to produce nano-scale islands of reproducible height, and the islands have been previously shown to effect cell events such as adhesion, spreading, proliferation, and differentiation. This study uses demixed poly(styrene) and poly(n-butyl methacrylate) to produce nano-islands with closer packing and narrower widths compared with those previously studied. Observations have been made of morphological and cytoskeletal changes in human fibroblasts interacting with 10- and 50-nm-high islands. The methods used included scanning electron microscopy, fluorescent microscopy, and optical microscopy. The results indicated that the cells do not respond differently to the 10-nm islands compared with planar samples but, in contrast, the 50-nm islands are nonadhesive.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP24936503	Poly(4-bromostyrene)		24936-50-3	Price

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