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Noninvasive Photoacoustic and Fluorescent Tracking of Optical Dye Labeled T Cellular Activities of Diseased Sites at New Depth

Noninvasive Photoacoustic and Fluorescent Tracking of Optical Dye Labeled T Cellular Activities of Diseased Sites at New Depth

Shuai Zheng, Honghui Li, Kejiong Lai, Maomao Chen, Guofeng Fu, Wen-Hsien Liu, Guo Fu, Liming Nie

J Biophotonics. 2018 Sep;11(9):e201800073.

PMID: 29701012

Abstract:

The migration of immune cells is crucial to the immune response. Visualization of these processes has previously been limited because of the imaging depth. We developed a deep-penetrating, sensitive and high-resolution method to use fast photoacoustic tomography (PAT) to image the dynamic changes of T cells in lymph node and diseases at new depth (up to 9.5 mm). T cells labeled with NIR-797-isothiocyanate, an excellent near-infrared photoacoustic and fluorescent agent, were intravenously injected to the mice. We used fluorescence imaging to determine the location of T cells roughly and photoacoustic imaging is used to observe T-cell responses in diseased sites deeply and carefully. The dynamic changes of T cells in lymph node, acute disease (bacterial infection) and chronic disease (tumor) were observed noninvasively by photoacoustic and fluorescence imaging at different time points. T cells accumulated gradually and reached a maximum at 4 hours and declined afterwards in lymph node and bacterial infection site. At tumor model, T cells immigrated to the tumor with a maximum at 12 hours. Our study can not only provide a new observing method for immune activities tracking, but also enable continuous monitoring for therapeutic interventions.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP152111916	NIR-797 isothiocyanate		152111-91-6	Price

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