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Normoxic Lung ischemia/reperfusion Accelerates Shedding of Angiotensin Converting Enzyme From the Pulmonary Endothelium

Normoxic Lung ischemia/reperfusion Accelerates Shedding of Angiotensin Converting Enzyme From the Pulmonary Endothelium

E N Atochina, V R Muzykantov, A B Al-Mehdi, S M Danilov, A B Fisher

Am J Respir Crit Care Med. 1997 Oct;156(4 Pt 1):1114-9.

PMID: 9351610

Abstract:

Normoxic lung ischemia/reperfusion (I/R) leads to oxidative injury of the pulmonary tissue. We analyzed angiotensin-converting enzyme (ACE) in perfused rat lungs upon I/R in order to assess the endothelial injury produced. I/R led to a time-dependent increase in ACE activity in the perfusate, from 145+/-14 mU to 252+/-1 mU, and to reduction of ACE activity in the lung tissue homogenate, from 29.7+/-2.3 U to 22.7+/-1.7 U. About 80% of ACE activity in control and I/R rat lungs was associated with an aqueous phase of extracted perfusates, thus indicating that I/R accelerates shedding of the hydrophilic form of ACE from the plasma membrane. To specifically assess ACE localized on the luminal surface of the pulmonary endothelium, we perfused rat lungs with a radiolabeled monoclonal antibody (mAb) to ACE (anti-ACE mAb 9B9). Pulmonary uptake of mAb 9B9 with I/R was reduced from 32.1+/-1.7% to 24.8+/-0.9%. In contrast, I/R led to a marked increase in the pulmonary uptake of nonspecific [125I]IgG, from 0.17+/-0.02% to 0.67+/-0.04%. Lung wet weight was equal to 0.78+/-0.08% of body weight in the I/R group versus 0.57+/-0.02% at the control level. The observed increase in [125I]IgG uptake and wet lung weight indicate that I/R causes an increase in lung vascular permeability. These results indicate that normoxic lung I/R induces injury to the pulmonary vascular endothelium.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248225	Monoclonal Anti-ACE antibody produced in mouse			Price

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