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Novel, Biocatalytically Produced Hydroxytyrosol Dimer Protects Against Ultraviolet-Induced Cell Death in Human Immortalized Keratinocytes

Novel, Biocatalytically Produced Hydroxytyrosol Dimer Protects Against Ultraviolet-Induced Cell Death in Human Immortalized Keratinocytes

Refiloe E Zwane, Ayesha Parker, Tukayi Kudanga, Lester M Davids, Stephanie G Burton

J Agric Food Chem. 2012 Nov 21;60(46):11509-17.

PMID: 23072558

Abstract:

Compounds derived from botanicals, such as olive trees, have been shown to possess various qualities that make them function as ideal antioxidants and, in doing so, protect them against the damaging effect of ultraviolet (UV)-derived oxidative stress. The aim of this study was to biocatalytically synthesize a dimeric product (compound II) from a known botanical, 3-hydroxytyrosol, and test it for its antioxidant ability using a human immortalized keratinocyte cell line (HaCaT). 2,2-Diphenyl-picryhydrazyl (DPPH) antioxidant assays showed 33 and 86.7% radical scavenging activity for 3-hydroxytyrosol and its dimer, respectively. The ferric-reducing antioxidant power (FRAP) assay corroborated this by showing a 3-fold higher antioxidant activity for the dimer than 3-hydroxytyrosol. Western blot analyses, showing cells exposed to 500 μM of the dimeric product when ultraviolet A (UVA)-irradiated, increased the anti-apoptotic protein Bcl-2 expression by 16% and reduced the pro-apoptotic protein Bax by 87.5%. Collectively, the data show that the dimeric product of 3-hydroxytyrosol is a more effective antioxidant and could be considered for use in skin-care products, health, and nutraceuticals.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP10597601-A	3-Hydroxytyrosol		10597-60-1	Price

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