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Novel Block Copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA Uptake and Cell Transfection

Novel Block Copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA Uptake and Cell Transfection

Shanta Raj Bhattarai, Ho-Keun Yi, Narayan Bhattarai, Pyoung Han Hwang, Hak Yong Kim

Acta Biomater. 2006 Mar;2(2):207-12.

PMID: 16701879

Abstract:

The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was significantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-co-L-lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the beta-galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantified by beta-galactosidase assay and spectrofluorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could find application in gene therapy.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS74399	Poly(p-dioxanone-co-L-lactide)			Price

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