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Novel Spectrofluorimetric Method for the Determination of Perindopril Erbumine Based on Charge Transfer Reaction With 7-Hydroxycoumarin

Novel Spectrofluorimetric Method for the Determination of Perindopril Erbumine Based on Charge Transfer Reaction With 7-Hydroxycoumarin

Hanan Fael, Amir Al-Haj Sakur

J Fluoresc. 2015 Jul;25(4):811-8.

PMID: 26149499

Abstract:

A novel, simple, selective and sensitive spectrofluorimetric method was developed and validated for the determination of perindopril erbumine using 7-hydroxycoumarin. Perindopril erbumine was found to react with 7-hydroxycoumarin in acetonitrile resulting in a new fluorescent product with about 58 nm blue shifted emission. The fluorescence of the complex was measured at 440 nm after excitation at 350 nm in acetonitrile. Under the optimum conditions, the fluorescence intensity was linear over a concentration range of 2.0-16.0 μg/mL (R(2) = 1) with a detection limit of 0.054 μg/mL. The proposed method was fully validated and successfully applied to the analysis of perindopril erbumine in pure form and tablets. Statistical comparison of the results obtained by the proposed and reference method revealed no significant differences in the performance of the two methods regarding the accuracy and precision respectively. The method was shown to be highly specific in the presence of indapamide, a diuretic that is commonly combined with perindopril erbumine. A proposal for the reaction pathway with 7-hydroxycoumarin was postulated.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP107133368	Perindopril erbumine		107133-36-8	Price

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