Home
Resources
Publications
NP603, a Novel and Potent Inhibitor of FGFR1 Tyrosine Kinase, Inhibits Hepatic Stellate Cell Proliferation and Ameliorates Hepatic Fibrosis in Rats

NP603, a Novel and Potent Inhibitor of FGFR1 Tyrosine Kinase, Inhibits Hepatic Stellate Cell Proliferation and Ameliorates Hepatic Fibrosis in Rats

Nan Lin, Si Chen, Weidong Pan, Linan Xu, Kunpeng Hu, Ruiyun Xu

Am J Physiol Cell Physiol. 2011 Aug;301(2):C469-77.

PMID: 21543745

Abstract:

Fibroblast growth factor 2 (FGF-2) and its main receptor FGFR1 have been shown to promote hepatic stellate cell (HSC) activation and proliferation. However, scant information is available on the anti-fibrogenic activity of FGFR1 inhibitors. The aim of this study was to assess the impact of a selective FGFR1 tyrosine kinase inhibitor NP603 on HSC proliferation and hepatic fibrosis. We demonstrated that rat primary HSCs secreted significant amounts of FGF-2, and its tyrosine phosphorylation of FGFR1 was attenuated by NP603. NP603 inhibited HSC activaton by measuring the expression of α-smooth muscle actin (α-SMA) and the production of type I collagen using ELISA. Furthermore, NP603 (25 μM) in vitro strongly suppressed HSC growth induced by FGF-2 (10 ng/ml) and FCS. This effect correlated with the suppression of extracellular-regulated kinase (ERK) activity and its downstream targets cyclin D1 and p21. In addition, PO NP603 (20 mg·kg(-1)·day(-1)) administration significantly decreased hepatic collagen deposition and α-SMA expression in CCl(4)-treated rats. Collectively, these studies suggest that selective blocking of the FGFR1-mediated pathway could be a promising therapeutic approach for the treatment of hepatic fibrosis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP949164801-B	NP603		949164-80-1	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: