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One- And Two-Dimensional Direct Chiral Liquid Chromatographic Determination of Mixtures of diclofop-Acid and Diclofop-Methyl Herbicides

One- And Two-Dimensional Direct Chiral Liquid Chromatographic Determination of Mixtures of diclofop-Acid and Diclofop-Methyl Herbicides

Vanesa Guillén-Casla, Luis Vicente Pérez-Arribas, María Eugenia León-González, Luis María Polo-Díez

J Agric Food Chem. 2008 Apr 9;56(7):2303-9.

PMID: 18338866

Abstract:

Simple one- and two-dimensional high-performance liquid chromatography (HPLC) methods for the simultaneous enantiomeric determination of alkyloxyphenoxypropionic acid herbicides is presented. Compounds studied were ( R, S)-2-[4-(2,4-dichlorophenoxy)phenoxy]propionic acid (diclofop-acid) and ( R, S)-2-[4-(2,4-dichlorophenoxy)]methyl propionate (diclofop-methyl). Mobile phases necessary to separate their enantiomers on an alpha1-acid glycoprotein chiral stationary phase are different; therefore, the simultaneous separation by an isocratic mode is not possible. The chiral separation method proposed involves a one-step gradient allowing for the simultaneous determination of both racemic enantiomers. Detection limits of the method were 0.03 mg/L for both diclofop-acid enantiomers and 0.14 and 0.15 mg/L for diclofop-methyl enantiomers, respectively. The two-dimensional method involves the use of two chromatographs in one achiral-chiral coupling. The LC-LC method is more suitable for complex samples because it involves an online cleanup effect. Detection limits were 1.25 and 1.87 mg/L for diclofop-acid and 2.70 and 3.02 mg/L for diclofop-methyl enantiomers, respectively. Accuracy, repeatability, and reproducibility have been studied in standard samples and a technical product.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP40843252	(±)-Diclofop		40843-25-2	Price

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