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One-dimensional SDS-polyacrylamide Gel Electrophoresis (1D SDS-PAGE)

Julie L Brunelle, Rachel Green

Methods Enzymol. 2014;541:151-9.

PMID: 24674069

Abstract:

This protocol describes a denaturing polyacrylamide gel system utilizing sodium dodecyl sulfate (SDS) to separate protein molecules based on size as first described by Laemmli (1970). SDS-PAGE can be used to monitor protein purifications, check the purity of samples, and to estimate molecular weights for unknown proteins.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP9003058-B Polyacrylamide Polyacrylamide 9003-05-8 Price
IAR424674 Fluorescein-α-D-galactosamine polyacrylamide Fluorescein-α-D-galactosamine polyacrylamide Price
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