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One-step Purification of Vitronectin From Human Plasma by Affinity Chromatography on Phage-Displayed Peptides

Szymon Skurzynski

Acta Biochim Pol. 2010;57(1):89-93.

PMID: 20349026

Abstract:

A novel affinity purification method for rapid isolation of vitronectin (VN) from human plasma is described. Recently we have used phage display technology to obtain clones expressing peptides with high binding activity for VN. The isolated "strong VN binders" were covalently coupled to CNBr-activated Sepharose. Human plasma was applied to the column and bound VN was eluted using 0.5 M acetic acid, giving purity exceeding 90%. The developed method is a convenient alternative to conventional antibody-antigen affinity chromatography techniques for purification of VN, as it offers low ligand cost, is rapid and ensures good protein recovery from human plasma.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP83380829-B Vitronectin from human plasma Vitronectin from human plasma 83380-82-9 Price
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