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One-step Separation of Four Flavonoids From Herba Salviae Plbeiae by HSCCC

One-step Separation of Four Flavonoids From Herba Salviae Plbeiae by HSCCC

Jia Li, Xiaoyong Zhang, Qi Yu, Xiaohui Fu, Weina Wang

J Chromatogr Sci. Nov-Dec 2014;52(10):1288-93.

PMID: 24615721

Abstract:

High-speed countercurrent chromatography (HSCCC) was successfully applied to the isolation and purification of four flavonoids from Herba Salviae Plbeiae using stepwise and dual-mode elution with a pair of two-phase solvent systems. The systems composed of n-hexane-chloroform-methanol-water (0.5 : 4 : 3 : 2, v/v/v/v) and chloroform-methanol-water (4 : 3 : 2, v/v/v). Analytical HSCCC was used for the preliminary selection of a suitable system, and a linear scale-step procedure was performed at a preparative grade. Hispidulin (91 mg), nepetin (148 mg), homoplantaginin (405 mg) and nepetin-7-glucoside (192 mg) could be obtained from 1,500-mg crude sample in a one-step separation, with purities >95% as determined by high-performance liquid chromatography. Their chemical structures were identified by (1)H nuclear magnetic resonance (NMR) and (13)C NMR.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP569904	Nepetin 7-glucoside		569-90-4	Price

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