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Ophiopogonin-D Suppresses MDA-MB-435 Cell Adhesion and Invasion by Inhibiting Matrix metalloproteinase-9

Ophiopogonin-D Suppresses MDA-MB-435 Cell Adhesion and Invasion by Inhibiting Matrix metalloproteinase-9

Yuanyuan Zhang, Yuwei Han, Kefeng Zhai, Minhui Sun, Jihua Liu, Boyang Yu, Junping Kou

Mol Med Rep. 2015 Jul;12(1):1493-8.

PMID: 25816153

Abstract:

Ophiopogonin-D is one of steroidal saponins isolated from the root of the Chinese medicinal plant Ophiopogon japonicas. It has been claimed to possess anti-inflammatory and anti-oxidant properties. The present study was the first to examine the anti-tumor metastasis properties of ophiopogonin-D. An MTT assay showed that ophiopogonin-D inhibited the proliferation of MDA-MB-435 melanoma cells, and decreased invasion was demonstrated using a Transwell invasion assay. Furthermore, adhesion of MDA-MB-435 cells to human umbilical vascular endothelial cells and to fibronectin was inhibited by ophiopogonin-D. Gelatin zymography and western blot analysis showed that ophiopogonin-D inhibited the expression and secretion of matrix metalloproteinase-9 (MMP-9), but not that of MMP-2. Inhibition of phosphorylation of p38 by ophiopogonin-D indicated its inhibition of the mitogen-activated protein kinase pathway. Overall, the results suggested that ophiopogonin-D may be considered as a candidate drug for treating or preventing tumor metastasis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP945619749	Ophiopogonin D		945619-74-9	Price

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