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Optimization and Partial Characterization of Ca-Independent α-amylase From Bacillus Amyloliquefaciens BH1

Optimization and Partial Characterization of Ca-Independent α-amylase From Bacillus Amyloliquefaciens BH1

Renpeng Du, Fangkun Zhao, Xiaoxiao Qiao, Qiaozhi Song, Guangbin Ye, Yu Wang, Binbin Wang, Ye Han, Zhijiang Zhou

Prep Biochem Biotechnol. 2018;48(8):768-774.

PMID: 30303444

Abstract:

Strain Bacillus amyloliquefaciens BH1 was evaluated for the generation of α-amylase. Culture conditions and medium components were optimized by a statistical approach for the optimal generation of α-amylase with response surface methodology (RSM) method. The Plackett-Burman (PB) design was executed to select the fermentation variables and Central composite design (CCD) for optimizing significant factors influencing production. The optimum levels for highest generation of α-amylase activity (198.26 ± 3.54 U/mL) were measured. A 1.69-fold improve generation was acquired in comparison with the non-optimized. Partial characterization of the α-amylase indicated optimal pH and temperature at 7.0 and 40 °C, respectively. Crude α-amylase maintained a constant pH range 5.0-8.0 and 30-70 °C. The α-amylase was independent of Ca2+, and the activity was inhibited by Fe3+, Co2+, Cu2+, and Hg2+. The thermo and pH stability of the α-amylase indicate its extensive application in the food and pharmaceutical industries.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9000855-B	α-Amylase from Bacillus amyloliquefaciens		9000-85-5	Price

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